Abbababa

Available from version 0.559+.

performs the abbababa test also called the D-statistic. This tests for ancient admixture (or wrong tree topology)

<classdiagram type="dir:LR">

[BAM files{bg:orange}]->[Sequence data|Random base]

[sequence data]->[*.abbababa|ABBA and BABA couts file{bg:blue}] </classdiagram>

<classdiagram type="dir:LR"> [*.abbababa|ABBA and BABA couts file{bg:blue}]->jackKnife.R[D stat and Z scores{bg:blue}] </classdiagram>

Brief Overview

> ./angsd -doAbbababa

--------------
analysisAbbababa.cpp:
	-doAbbababa	0
	1: use a random base
	-rmTrans		0	remove transitions
	-blockSize		5000000	number of based in a block

This function will counts the number of ABBA and BABA sites

Options

-doAbbababa 1

sample a random base at each position.

-rmTrans

Remove transitions (important for ancient DNA)

-blockSize [INT]

Size of each block. Choose a number that is higher than the LD in the populations. For human 5Mb (5000000) is usually used.

-anc [fileName.fa]

Include an outgroup in fasta format.

-doCounts 1

use -doCounts 1 in order to count the bases at each sites after filters.

Output

• .abbbababa

Output: Each lines represents a block with a chromsome name (Column 1), a start position (Column 2), an end postion (Column 3). The new columns are the counts of ABBA and BABA sites. For each combination of 3 individuals (H1,H2,H3) two columns are printed. These number served as input to the R script called jackKnife.R

Example

Create a fasta file bases from a random samples of bases.

head -n5 smallBam.filelist > smallerBam.filelist
./angsd -out out -doAbbababa 1 -bam smallerBam.filelist -doCounts 1 -anc /space/genomes/refgenomes/ancestral/hg19/fasta/hg19ancNoChr.fa
Rscript R/jackKnife.R file=out.abbababa indNames=smallerBam.filelist outfile=out

This results in a out.txt file with all the results.

output

H1	H2	H3	 nABBA	nBABA	Dstat	jackEst	SE	Z	
NA11830	NA12004	NA12763	269	322	-0.08967851	-0.08967851	0.09006086	-0.9957545	
NA11830	NA06985	NA12763	267	298	-0.05486726	-0.05486726	0.122256	-0.4487898	
NA12004	NA06985	NA12763	254	243	0.0221328	0.0221328	0.1386198	0.1596655	
NA11830	NA11993	NA12763	225	336	-0.197861	-0.197861	0.08514797	-2.323731	
NA12004	NA11993	NA12763	217	267	-0.1033058	-0.1033058	0.09471542	-1.090697	
NA06985	NA11993	NA12763	242	302	-0.1102941	-0.1102941	0.1241554	-0.8883553	
NA12763	NA12004	NA11830	237	322	-0.1520572	-0.1520572	0.1047361	-1.451813	
NA12763	NA06985	NA11830	219	298	-0.1528046	-0.1528046	0.1115283	-1.370098	

H1 H2 H3 are the 3 individuals in the tree that are not the outgroup. H1 and H2 are the ingroup see tree

nABBA the total counts of ABBA patterns

nBABA the total counts of BABA patterns

Dstat The test statistic

(nABBA-nBABA)/(nABBA+nBABA). A negative value means that H1 is closer to H3 than H2 is. A positive value means that H2 is closer to H3 than H1 is.

JackEst column is another estimate of the abbababa statistic that is bias corrected. This value is extremely similar to the value in the Dstat column

SE is the estimated m-delete blocked Jackknife Standard error of the estimate used to obtain the Z value

Z Z value that can be used to determine the significance of the test. As in Reich et al. an absolute value of the Z score above 3 is often used as a critical value. However, this note that this does not take into account the fact that we perform multiple tests.