ANGSD: Analysis of next generation Sequencing Data
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Fasta: Difference between revisions
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./angsd -i smallNA07056.mapped.ILLUMINA.bwa.CEU.low_coverage.20111114.bam -doFasta 1 | ./angsd -i smallNA07056.mapped.ILLUMINA.bwa.CEU.low_coverage.20111114.bam -doFasta 1 | ||
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Revision as of 17:44, 27 November 2013
This option create a fasta file from a sequencing data file. The function uses genome information in the bam header to determing the length and chromosome names. For the sites without data an "N" is written.
<classdiagram type="dir:LR">
[One bam file{bg:orange}]->[sequencing data|random base (-doFasta 1);consensus base (-doFasta 2)]
[sequencing data]->doFasta[fasta file{bg:blue}]
</classdiagram>
Brief Overview
> ./angsd -doFasta -------------- analysisFasta.cpp: -doFasta 0 1: use a random base 2: use the most common base (needs -doCounts 1) -minQ 13 (remove bases with qscore<minQ)
options
- -doFasta 1
- sample a random base
- -doFasta 2
- use the most common base. In the case of ties a random base is chosen among the bases with the same counts
- -minQ [INT]
minimum base quality score
Example
Create a fasta file bases on a random samples of bases
./angsd -i smallNA07056.mapped.ILLUMINA.bwa.CEU.low_coverage.20111114.bam -doFasta 1