ANGSD: Analysis of next generation Sequencing Data

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Fasta: Difference between revisions

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;-doFasta 1: sample a random base
;-doFasta 1: sample a random base


;-doFasta 2: use the most common base. In the case of ties a random base is chosen among the bases with the same counts  
;-doFasta 2: use the most common base. In the case of ties a random base is chosen among the bases with the same counts. The "-doCounts 1" options for [[Alleles_counts|allele counts]] is needed in order to determine the most common base


;-minQ [INT]  
;-minQ [INT]  

Revision as of 17:47, 27 November 2013

This option create a fasta file from a sequencing data file. The function uses genome information in the bam header to determing the length and chromosome names. For the sites without data an "N" is written.

<classdiagram type="dir:LR">

[One bam file{bg:orange}]->[sequencing data|random base (-doFasta 1);consensus base (-doFasta 2)]

[sequencing data]->doFasta[fasta file{bg:blue}]

</classdiagram>

Brief Overview

> ./angsd -doFasta
--------------
analysisFasta.cpp:
	-doFasta	0
	1: use a random base
	2: use the most common base (needs -doCounts 1)
	-minQ		13	(remove bases with qscore<minQ)

options

-doFasta 1
sample a random base
-doFasta 2
use the most common base. In the case of ties a random base is chosen among the bases with the same counts. The "-doCounts 1" options for allele counts is needed in order to determine the most common base
-minQ [INT]

minimum base quality score


Example

Create a fasta file bases on a random samples of bases

./angsd -i smallNA07056.mapped.ILLUMINA.bwa.CEU.low_coverage.20111114.bam -doFasta 1