Revision as of 15:20, 17 July 2013

This will contain the program called NGSadmix, which is a very nice tool for finding admixture. It is based on genotype likelihoods. It is a fancy multithreaded c/c++ program.

Latest version is 32 from June 25 2013. It can be found [1]. Older versions can be found here: [2]

Installation

wget popgen.dk/software/NGSadmix/ngsadmix32.cpp 
g++ ngsadmix32.cpp -O3 -lpthread -lz -o NGSadmix

Run example

Assuming we have an input file called input.gz and we assume 3 ancestral populations (-K 3), and that we want to use 4 computing cores (-P 4). The prefix of the output files is myoutfiles (-o myoutfiles) using only SNP with af MAF above 5% (-minMaf 0.05 )

./NGSadmix -likes input.gz -K 3 -P 4 -o myoutfiles -minMaf 0.05

Get example data from the 1000 genomes project

wget popgen.dk/software/NGSadmix/data/input.gz
wget popgen.dk/software/NGSadmix/data/pop.info

Input Files

Input files are contains genotype likelihoods in genotype likelihood beagle input file format [3]. We recommend ANGSD for easy transformation of Next-generation sequencing data to beagle format.

example of a beagle genotype likelihood input file for 3 individuals.

marker       allele1  allele2   Ind0      Ind0    Ind0     Ind1     Ind1     Ind1     Ind2     Ind2     Ind2
1_14000023      1       0       0.941    0.058    0.000    0.799    0.199    0.001    0.666    0.333    0.001 
1_14000072      2       3       0.709    0.177    0.112    0.941    0.058    0.000    0.665    0.332    0.001
1_14000113      0       2       0.855    0.106    0.037    0.333    0.333    0.333    0.799    0.199    0.000 
1_14000202      2       0       0.835    0.104    0.060    0.799    0.199    0.000    0.333    0.333    0.333
...

column 1:The marker name (the information is not atually used)

column 2 and 3: the major and minor alle (the information is not atually used)

The rest of the colums are the genotypes likelihoods (not in log space). For each individual 3 coloums are needed. Note that the above values sum to one per sites for each individuals. This is just a normalization of the genotype likelihoods in order to avoid underflow problems in the beagle software it does not mean that they are genotype probabilities.

The file is allowed to be compressed using gzip

Options

./NGSadmix
Arguments:
	-likes Beagle likelihood filename
	-K Number of ancestral populations
Optional:
	-fname Ancestral population frequencies
	-qname Admixture proportions
	-o Prefix for output files
	-printInfo print ID and mean maf for the SNPs that were analysed
Setup:
	-seed Seed for initial guess in EM
	-P Number of threads
	-method If 0 no acceleration of EM algorithm
	-misTol Tolerance for considering site as missing
Stop chriteria:
	-tolLike50 Loglikelihood difference in 50 iterations
	-tol Tolerance for convergence
	-dymBound Use dymamic boundaries (1: yes (default) 0: no)
	-maxiter Maximum number of EM iterations
Filtering
	-minMaf Minimum minor allele frequency
	-minLrt Minimum likelihood ratio value for maf>0
	-minInd Minumum number of informative individuals

Output Files

Program outputs 3 files.

PREFIX.log
PREFIX.fopt.gz
PREFIX.qopt

The log file contains log information of the run. Commandline used for running the program, what the likelihood is every 50 iterations, and finally how long it took to do the run.

The fopt.gz file is an compressed file, which contains an estimate of the frequency for each site for all populations.

The qopt file contains the admixture proportions for all individuals.

Examples of the output files are found below.

Log file

Contents of the file log file

	-> Dumping file: tskSim/tsk6GL.beagle.s1.log
	-> Dumping file: tskSim/tsk6GL.beagle.s1.filter
Input: lname=tskSim/tsk6GL.beagle nPop=3, fname=(null) qname=(null) outfiles=tskSim/tsk6GL.beagle.s1
Setup: seed=1 nThreads=10 method=1
Convergence: maxIter=2000 tol=0.000000 tolLike50=0.010000 dymBound=0
Filters: misTol=0.050000 minMaf=0.000000 minLrt=0.000000 minInd=0
Input file has dim: nsites=100000 nind=75
Input file has dim (AFTER filtering): nsites=100000 nind=75
iter[start] like is=9299805.984931
iter[50] like is=-6531138.892608 thres=0.002800
iter[100] like is=-6528710.773349 thres=0.001289
iter[150] like is=-6528405.896951 thres=0.001211
iter[200] like is=-6528306.803820 thres=0.000420
iter[250] like is=-6528277.160993 thres=0.000546
iter[300] like is=-6528271.925055 thres=0.000033
iter[350] like is=-6528271.177692 thres=0.000008
iter[400] like is=-6528270.876315 thres=0.000005
iter[450] like is=-6528270.772894 thres=0.000140
iter[500] like is=-6528270.747721 thres=0.000002
iter[550] like is=-6528270.740654 thres=0.000002
Convergence achived because log likelihooditer difference for 50 iteraction is less than 0.010000
best like=-6528270.740654 after 550 iterations
	-> Dumping file: tskSim/tsk6GL.beagle.s1.qopt
	-> Dumping file: tskSim/tsk6GL.beagle.s1.fopt.gz
	[ALL done] cpu-time used =  671.82 sec
	[ALL done] walltime used =  114.00 sec

Allele frequency ouput (.fopt)

Each column correponds to the estimated allele frequencies for each population and each line is a SNP

Example of a .fopt file for -K 3

...
0.75331646167520038837 0.51190946588401886608 0.50134051056701267601 
0.99999999900000002828 0.80165850924934911603 0.97470665326916294813 
0.99999999900000002828 0.89560828888972687789 0.88062641752218895341 
0.99999999900000002828 0.99999999900000002828 0.86109994249930577048 
0.70560445653074521655 0.78994686954000448154 0.93076614062025020413 
0.99999999900000002828 0.88878537780630872955 0.92662857068149151463 
0.05322676762098016434 0.22871739860812340117 0.17394852600322696645 
0.00000000100000000000 0.27428885137150410545 0.19029599645013275944 
0.57086006389212373691 0.42232596591112880891 0.74080063581586474974 
0.77359733910003525281 0.47380864146016693494 0.72073560889718923939 
0.49946404159405927148 0.21684946347150244050 0.15201985942558055021 
0.41802171086717271331 0.55490556205954566504 0.85691127728452165524 
0.77095213528720529794 0.60074618451005279418 0.70219544996184157792 
0.26517850405564091787 0.48500265408436060710 0.85432254709914456914 
0.80055081986260245852 0.74423201242010783574 0.87110476762969968334 
0.30563054476851375663 0.05233529475348827620 0.25911912824038613179 
0.51084997710733415222 0.62263692178557350498 0.50738250264097506381 
0.64790272562679740442 0.91230541484222271720 0.73015721390331478347 
0.07124629651164265942 0.37896482494356753534 0.29218012479334326548 
0.00000000100000000000 0.26969100790961914038 0.28395781874856029781 
0.97074775756045073027 0.79093498372643300520 0.64006920058897498471 
0.64661948716978157048 0.84130009558421925409 0.76730057769159087933 
0.86990900887920663553 0.79410745692063922085 0.69416721874359499367 
0.34956069940263900797 0.27773038429396151860 0.25923476721423144298 
0.77739744690560164120 0.51272232330145017798 0.53888718200036844763 
0.35431569298041332150 0.20022780744715171219 0.43176580786072032980 
0.91858160919413811563 0.99999999900000002828 0.93584179237779097082 
0.90339823126358831384 0.94729687041528465308 0.84358671720630329371 
0.87068129661127857677 0.65267891763324525911 0.59315740612546075106 
0.24102496839012735319 0.42777100607917967201 0.39594098602469629533 
0.99999999900000002828 0.99999999900000002828 0.78549330115836857313 
0.15386277372522660922 0.18035502891341426146 0.26583557049163752950 
0.22456748943597096280 0.25110807159057474403 0.17244618960511531869 
0.74816053649164548922 0.54769319158907958656 0.44532166240679449398 
0.76350303696805599252 0.86547244122202959815 0.94111974586621383043 
0.40940400475566068872 0.67767095908245833513 0.40793761498610620064 
0.85389765162910868934 0.78901563183853873351 0.93614065916219291186 
0.54108661985898742763 0.61895909938546000983 0.88522763262549941654 
0.99051495581855464323 0.78855843624128341141 0.77646441702623147929 
0.51133721761171413434 0.74521610846562824637 0.32689774480116673416 
0.66618479413060949224 0.67891474309775079465 0.80762116232856140385 
0.81793598261160704865 0.77752326447671193943 0.95349025244041396565 
0.82120324647844433752 0.99999999900000002828 0.89800731971059466474 
...

Use the -printInfo option to get the position of the lines in the fopt file if some sites have been flltered from the analysis (-minMaf, minInd, minLRT etc)

Admixture proportion output file (-qopt)

Infered admixture proporsions. Each line is an individual and each column is a population.

Contents of the qopt file # cat tsk48GL.beagle.gz.s1.qopt

0.00254460532103031574 0.00108987228478324210 0.99636552239418640919 
0.00000015905647541105 0.00000000100000000000 0.99999983994352459327 
0.00034770382567266174 0.02639209238328452459 0.97326020379104283275 
0.00000000100000000000 0.00000000100000000000 0.99999999800000005656 
0.00000467398081877176 0.00000000100000000000 0.99999532501918120264 
0.00000000907496942853 0.00585150933779484805 0.99414848158723567728 
0.00515826525767644137 0.01138897436535154552 0.98345276037697204607 
0.03914841746468285949 0.00000000100000000000 0.96085158153531713410 
0.00000000100000000000 0.00629199375758324100 0.99370800524241675866 
0.00771173022930659625 0.00000154720357311662 0.99228672256712036059 
0.00000000100000000000 0.00075135345721917719 0.99924864554278081119 
0.00000000100000000000 0.00000000100000000000 0.99999999799999994554 
0.00000005468413042120 0.00087279924180633879 0.99912714607406327705 
0.00000000100000000000 0.00000000100000000000 0.99999999800000005656 
0.00712941313019542066 0.00118955677574110528 0.99168103009406338710 
0.00000000100000000000 0.00000000100000000000 0.99999999799999994554 
0.00000000100000000000 0.00165385222968000606 0.99834614677032007535 
0.00000000100000000000 0.00006297763597355473 0.99993702136402651259 
0.00519087111391381209 0.00000000100000000000 0.99480912788608621966 
0.00000000100000000000 0.00000000100000000000 0.99999999800000005656 
0.00202872783596746379 0.00000000100000000000 0.99797127116403261393 
0.00876424336999809782 0.00949457841911990376 0.98174117821088191516 
0.00000000100000000000 0.00000000100000000000 0.99999999799999994554 
0.00000000100000000000 0.00000000100000000000 0.99999999799999994554 
0.00000000100000000000 0.00000000100000000000 0.99999999799999994554 
0.00000000100000000000 0.00000000100000000000 0.99999999799999994554 
0.00000000100000000000 0.00000000100000000000 0.99999999800000005656 
0.01820430093358888640 0.00000694033297829119 0.98178875873343274261 
0.00351013812443964728 0.00000020340562512923 0.99648965846993520223 
0.00771897550085272680 0.00605259705033356268 0.98622842744881378252 
0.00600595292580561029 0.00000000100000000000 0.99399404607419439284 
0.01454910070242997067 0.00543457657939076105 0.98001632271817917808 
0.02567862615486414535 0.00160921436783232220 0.97271215947730349516 
0.00000000100000000000 0.00000000100000000000 0.99999999800000005656 
0.00000000100000000000 0.00001041560507852223 0.99998958339492149960 
0.00000000100000000000 0.01383432553657116572 0.98616567346342876021 
0.00343840097404925389 0.00000000100000000000 0.99656159802595079000 
0.00000000100000000000 0.00000000100000000000 0.99999999800000005656 
0.00000000100000000000 0.00000000100000000000 0.99999999800000005656 
0.00051244065751142103 0.00404846039501185508 0.99543909894747661937 
0.02003953974792894652 0.00000004934009128878 0.97996041091197982897 
0.00000000100000000000 0.00000000100000000000 0.99999999799999994554 
0.00000000100000000000 0.00000000100000000000 0.99999999799999994554 
0.00000000100000000000 0.00000000100000000000 0.99999999800000005656 
0.00000000100000000000 0.00000000100000000000 0.99999999799999994554 
0.02176809890633762956 0.00000000100000000000 0.97823190009366245423 
0.00000000100000000000 0.00000000100000000000 0.99999999800000005656 
0.01563096189267457192 0.00970868396771427770 0.97466035413961116252 
0.00000000100000000000 0.00000000100000000000 0.99999999800000005656 
0.00002540964943070735 0.00000000100000000000 0.99997458935056915408 
0.99999999799999994554 0.00000000100000000000 0.00000000100000000000 
0.99501476026684787524 0.00000000100000000000 0.00498523873315206718 
0.99999999799999994554 0.00000000100000000000 0.00000000100000000000 
0.99520671498720802983 0.00479241730266987201 0.00000086771012207898 
0.95884374919730619435 0.00000000100000000000 0.04115624980269377842 
0.99002104218586972628 0.00000000100000000000 0.00997895681413022567 
0.99999999799999994554 0.00000000100000000000 0.00000000100000000000 
0.99999999800000005656 0.00000000100000000000 0.00000000100000000000 
0.99999999770925251941 0.00000000129074746013 0.00000000100000000000 
0.99999999799999994554 0.00000000100000000000 0.00000000100000000000 
0.99999999799999994554 0.00000000100000000000 0.00000000100000000000 
0.99999999800000005656 0.00000000100000000000 0.00000000100000000000 
0.99999999800000005656 0.00000000100000000000 0.00000000100000000000 
0.98980053177767901573 0.00000005577971952226 0.01019941244260143612 
0.99999999800000005656 0.00000000100000000000 0.00000000100000000000 
0.99999785004878083416 0.00000000100000000000 0.00000214895121910354 
0.99999999800000005656 0.00000000100000000000 0.00000000100000000000 
0.99999999800000005656 0.00000000100000000000 0.00000000100000000000 
0.99220030909132039820 0.00000000100000000000 0.00779968990867968733 
0.99999996788621803301 0.00000000100000000000 0.00000003111378189772 
0.99736783433174225344 0.00255940950853666971 0.00007275615972113173 
0.99998096423035520708 0.00000000574461213317 0.00001903002503262207 
0.99711097909957713270 0.00288887008493822353 0.00000015081548462101 
0.99999999799999994554 0.00000000100000000000 0.00000000100000000000 
0.99999999800000005656 0.00000000100000000000 0.00000000100000000000 
0.99999999800000005656 0.00000000100000000000 0.00000000100000000000 
0.99769262012085335734 0.00000000100000000000 0.00230737887914652393 
0.99999820787375570674 0.00000000433914936351 0.00000178778709493472 
0.98047422489554170166 0.00012980111977614777 0.01939597398468214523 
0.99999999799999994554 0.00000000100000000000 0.00000000100000000000 
0.98208006049140339488 0.00000000100000000000 0.01791993850859651197 
0.97530298545159921364 0.00000000100000000000 0.02469701354840085974 
0.99657542812406740840 0.00000000100000000000 0.00342457087593254226 
0.99954556420189066834 0.00045443479810919004 0.00000000100000000000 
0.99999999800000005656 0.00000000100000000000 0.00000000100000000000 
0.99531584565237773976 0.00410740812985130408 0.00057674621777084644 
0.99999999800000005656 0.00000000100000000000 0.00000000100000000000 
0.99878572597704817770 0.00000000100000000000 0.00121427302295177490 
0.98571687209123504125 0.00400077401169816448 0.01028235389706666329 
0.99027397554762419674 0.00840892511494516215 0.00131709933743062008 
0.99999993504923445631 0.00000000100000000000 0.00000006395076564386 
0.95946639819101930957 0.00000000100000000000 0.04053360080898076034 
0.99999999800000005656 0.00000000100000000000 0.00000000100000000000 
0.98414939425022363029 0.01585059024074651421 0.00000001550902978739 
0.99999999622245250297 0.00000000277754757396 0.00000000100000000000 
0.99525652466242930938 0.00000001683386219288 0.00474345850370842034 
0.99999999799999994554 0.00000000100000000000 0.00000000100000000000 
0.99999999799999994554 0.00000000100000000000 0.00000000100000000000 
0.99999965447943561792 0.00000000100000000000 0.00000034452056438734 
0.99864814059528783652 0.00135185840471215468 0.00000000100000000000 
0.00000000100000000000 0.99999999799999994554 0.00000000100000000000 
0.00000000100000000000 0.99999999800000005656 0.00000000100000000000 
0.00000001076370464123 0.99999998823629543399 0.00000000100000000000 
0.00000000100000000000 0.99999999799999994554 0.00000000100000000000 
0.00000000100000000000 0.99999999799999994554 0.00000000100000000000 
0.00000000099999999999 0.99999999799999994554 0.00000000099999999999 
0.00000000099999999999 0.99999999799999994554 0.00000000099999999999 
0.00000000100000000000 0.99999999800000005656 0.00000000100000000000 
0.00000000100000000000 0.99999999800000005656 0.00000000100000000000 
0.00000000100000000000 0.99999999799999994554 0.00000000100000000000 
0.00000000099999999999 0.99999999799999994554 0.00000000099999999999 
0.00000000099999999999 0.99999999800000005656 0.00000000099999999999 
0.00000000100000000000 0.99999999799999994554 0.00000000100000000000 
0.00000000100000000000 0.99999999799999994554 0.00000000100000000000 
0.00000000099999999999 0.99999999800000005656 0.00000000099999999999 
0.00000000100000000000 0.99999999799999994554 0.00000000100000000000 
0.00000000099999999999 0.99999999800000005656 0.00000000099999999999 
0.00000000100000000000 0.99999999800000005656 0.00000000100000000000 
0.00000000100000000000 0.99999999799999994554 0.00000000100000000000 
0.00000000100000000000 0.99999999800000005656 0.00000000100000000000 
0.00000000100000000000 0.99999999799999994554 0.00000000100000000000 
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0.00000000099999999999 0.99999999799999994554 0.00000000099999999999 
0.00000000100000000000 0.99999999799999994554 0.00000000100000000000 
0.00000000100000000000 0.99999999800000005656 0.00000000100000000000 
0.00000000100000000000 0.99999986659623718577 0.00000013240376283687 
0.00000000099999999999 0.99999999799999994554 0.00000000099999999999 
0.00000000099999999999 0.99999999799999994554 0.00000000099999999999 
0.00000000100000000000 0.99632783404679736705 0.00367216495320256799 
0.00000000100000000000 0.99999999799999994554 0.00000000100000000000 
0.00000000099999999999 0.99999999800000005656 0.00000000099999999999 
0.35919621347731411909 0.32381633362411937904 0.31698745289856661289 
0.31048363757756514136 0.30902410742704566893 0.38049225499538924522 
0.36341140678787386964 0.33678307361394943520 0.29980551959817652863 
0.34550713774447228133 0.34037087985425079628 0.31412198240127681137 
0.34705579219215104692 0.35218792485566730033 0.30075628295218165276 
0.33646039412306782967 0.32632754139618752598 0.33721206448074481088 
0.31881401220765009930 0.34885621407165418040 0.33232977372069577582 
0.34999374672052624424 0.33030931848049555066 0.31969693479897826061 
0.33152251818028721786 0.32339147992992234304 0.34508600188979043910 
0.31959998197389311025 0.33152491237148390413 0.34887510565462298562 
0.34724548642936803322 0.31809475756470984020 0.33465975600592196004 
0.33378069767858009609 0.33223636639277298599 0.33398293592864686241 
0.32023090400419051971 0.33179989332826043125 0.34796920266754916007 
0.35205158009776410521 0.33547091017851976558 0.31247750972371612921 
0.34291063455495451873 0.31853488093100223999 0.33855448451404313026 
0.31929132670383747472 0.32755905579808902717 0.35314961749807355362 
0.34114474726121107873 0.34607065583774476725 0.31278459690104404300 
0.33725705347681012025 0.32910919226619778089 0.33363375425699209886 
0.33918213722968154622 0.32278745806952213737 0.33803040470079642743 
0.33788659799509024317 0.34692305448657090317 0.31519034751833896468 
0.35876135180876589370 0.33843260979944000955 0.30280603839179404124 
0.34721570614318736370 0.34395335873604998556 0.30883093512076259524 
0.34165097731337079612 0.32814110943000784903 0.33020791325662146587 
0.33922542743931027864 0.32639619830977489867 0.33437837425091476717 
0.34461619391735059947 0.33133174331942943924 0.32405206276321996128 
0.34277551565686120716 0.32746953398981676342 0.32975495035332202942 
0.33842982221926010133 0.31224638933762871584 0.34932378844311123833 
0.34443810815667752490 0.32640113997211872565 0.32916075187120380496 
0.31723258569943768581 0.34955203711397470068 0.33321537718658750249 
0.35394053250677920408 0.33291498389624818444 0.31314448359697255597 
0.33504517457864940733 0.34188143503173562543 0.32307339038961496724 
0.33240938202788244960 0.34671459781042585080 0.32087602016169164409 
0.31745792352948248860 0.33722730677636020280 0.34531476969415725309 
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Plot results

Use R

admix<-t(as.matrix(read.table("myoutfiles.qopt")))
barplot(admix,col=1:3,space=0,border=NA,xlab="Individuals",ylab="admixture")

citation

log

v32 june 25-2013; modified code such that it now compiles on OSX
v31 june 24-2013; First public version.

NgsAdmix: Difference between revisions