RefFinder

Small fast cprogram to extract bases from a fasta file. Download here [1]

Program can either work as a standalone program, or allow for easy retrieval of reference bases by using the API.

Install

wget http://popgen.dk/software/download/refFinder/refFinder.tar.gz
tar xf refFinder.tar.gz
cd refFinder/
make
cd ..

Secret software

Grepper

Formerly known as getliner. Used for grep'ing with in columns

Sortdep

Fast counting of integers

RefFinder

Extract bases from a fasta file

Other stuff

chisquare

A small library build using numerical recipies third edition for calculating stuff relating to the chisqdistribution

bambi/bammer

(Thorfinn, april20 2012)

Download

bambi.tar.gz

Usage

Program is invoked with either:

./bammer view     #samtools view/ single sample only
./bammer uppile   #This does emulates the samtools mpileup

Inputfiles are supplied with either

tmp.bam                #single bamfile
-b bam.list            #filelist containing bamfiles
-b bam.list -nInd INT  #only select the first INT samples from bam.list

Regions of interest can be supplied by defining a region in the following way

-r chr:a-b  #select chromsome chr from a, to b
-r chr:-b   #select chromosome chr from beginning to be
-r chr:b-   #select chromosome chr from b to end of chromosome

If we want to dump different regions in the same run, this can be done with

-rf regions.file  #every region is on a newline, has same format as above

Program can also estimate genotypelikelihoods if uppile is selected

-GL 0    #SOAPsnp
-GL 1    #samtools
-GL 2    #gatk

Output is hardcode dumped in glfs.glfs, these is a binary double file. Information for first site is the first 10*sizeof(double)*nInd bytes. This is the samtools ordering AA,AC,AG,...

Information about the position is dumped in mafs.mafs. This is NOT the MAF, but simply a 2 column file with chr tab.

Below are some examples for running the program

#view commands below
./bammer view new2.bam
./bammer view bwape_sort_rmdup.bam chr21:9483252-9672320
./bammer view bwape_sort_rmdup.bam chrY:-1000000

#mpileup below
./bammer uppile -b lucampHighBam.filelist -nInd 2 -r chr1:-376920

#samtools estimation of GL
./bammer uppile -b lucampHighBam.filelist -nInd 2 -r chr1:-376920 -GL 1

Technics

Program spawns a single thread, when a chunk has been read, and the thread is not finished it will wait.

ieatgor

Extract lines from file in "gor" format. The files can be found in this [[ieatgor/][folder]]

to compile

g++ -O3 -o ieatgor ieatgor.cpp -lz

The target file must be sorted. example of target file

chr1:22323:232322
chr10:10000:10000000
chr3:10000:10000000
 

A "gor" file is a file with the first coloum being the chromosome and the second coloum being the positions. The rest of the coloums are not important. The gor file must be sorted based on the positions and chr. gz gor files are also accepted. example of a gor file

chr1    1033267 2       3       know 0.000000 EM 0.000000 unk 0.000000 EMunk 0.000000 Nind 9
chr1    1033268 0       2       know 0.000000 EM 0.000000 unk 0.000000 EMunk 0.000000 Nind 9
chr1    1033279 3       1       know 0.000000 EM 0.000000 unk 0.000000 EMunk 0.000000 Nind 9
chr1    1033286 0       2       know 0.000000 EM 0.000000 unk 0.000000 EMunk 0.000000 Nind 9
chr1    1033297 0       2       know 0.000000 EM 0.000000 unk 0.000000 EMunk 0.000000 Nind 9
chr1    1033304 0       1       know 0.000000 EM 0.000000 unk 0.000000 EMunk 0.000000 Nind 9
...
 

run example

./ieatgor file.target file.gor

the extracted line are returned to standard out.

getliner

NB HUGE BUG, must +1 when using -l and zipped files. Sorry for inconveincace. (tsk)

This is a general tool for extract lines from newline seperated textfiles (can be gz compressed). Getliners4 allows for complement grep, use '-v 1'. Default is '-v 0'. Program can also print out basic statistics for hit/nonhit if -i filename is supplied. getliners6.cpp tokinzes on \r, so should work on windows files.

Download here getliners6.cpp .

g++ getliners5.cpp -lz -O3 -o getliners

Program can either extract specific lines, or "keys". The linenumbers to extract must be 1-indexed (first line i 1). When using keys, you must supply which column to 'grep' for. The program builds an associative array of the keys. And therefore only a single pass of the datafile is required.

Fields in the datafile can be seperated by a delimiter that can be specified at runtine with the "-d " argument. Default is space/tab. If delimter is an escape sequence it will not work and the code should be modified.

calcABBABABA

This is program that calculates ABBA-BABA related stats for a given chromosome.

It can be downloaded here abbababatest.cpp and can be compiled with:

g++ -O3 abbababatest.cpp -o calcABBABABA -lz

To get all options just type

 ./calcABBABABA 

An example run cmd

../calcABBABABA -file /space/genomes/refgenomes/ancestal/hg19/shortForm/chr20.ans -bam  testbamfilelistbotoV2chr20.txt -outfileprefix testbotoV2Chr20plus1x2 -blocksz 5 

The program outputs three files: an abbababa file (with only abbababa stats), a count file (with overall counts for the chromosome) and a summary file where the compile time, run time and the cmds and the input files used when the program was run are all listed.

plot Plink

[[Rfun/][Folder]]

 Rscript /home/software/public/software/intern/Rfun/plink.plot.R <plink output file>

liftover a plink file

You might have the change the chainFile path and the liftover path if you are not on pontus Note that if two SNP map to the same position after liftover one of them is removed.

#!/bin/bash
#make liftover file
Files=$1
liftoverProg=/home/albrecht/bin/prog/liftover/liftOver
chainFile=/home/albrecht/bin/prog/liftover/chainFiles/hg18ToHg19.over.chain
plink=/home/albrecht/bin/prog/plink-1.07-x86_64/plink

if [ "$#" -eq 0 ]; then
    echo "supply a plink prefix"
    exit
fi

if [ -f $Files.map ]
then
    echo using file $Files.map
else
    echo the file ${Files}.map does not exists \(use plink --recode\)
fi

if [ -f $Files.bed ]
then
    echo using file $Files.bed
else
    echo the file $Files.bed does not exists  \(use plink --make-bed\)
fi
#make bed file for liftover
Rscript -e "options(scipen=10);map<-read.table('$Files.map',hea=F,as.is=T);res<-cbind(paste('chr',map[,1],sep=''),map[,4]-1,map[,4],map[,2],0,'+');write.table(res,file='$Files.bedfile',col=F,row=F,qu=F,sep='\t')"

#liftover
$liftoverProg $Files.bedfile $chainFile ${Files}Hg19.bedfile ${Files}Hg19.unmapped

# rm unmapped
grep -v \# ${Files}Hg19.unmapped  | cut -f4 > rmSNPs.txt
#rm duplicates
cat ${Files}Hg19.bedfile | sort -k1 | rev | uniq -d -f3 | rev | cut -f4 > dubSNP.txt
echo removing `wc -l dubSNP.txt`
cat dubSNP.txt >> rmSNPs.txt

$plink --noweb --bfile $Files --exclude rmSNPs.txt --recode --out ${Files}Red 
#change pos

Rscript -e "liftmap<-read.table('${Files}Hg19.bedfile',hea=F,colC=c('character','integer')[c(1,2,2,1,2,1)]);liftmap<-read.table('${Files}Hg19.bedfile',hea=F,as.is=T);map<-read.table('${Files}Red.map',hea=F,as.is=T); liftmap<-liftmap[liftmap[,4]%in%map[,2],];rownames(map)<-map[,2];map[liftmap[,4],4]<-liftmap[,3];map[liftmap[,4],1]<-sub('chr','',liftmap[,1]);write.table(map,file='hg19.map',col=F,row=F,qu=F)"

echo The new plink file is called  ${Files}Hg19 and are on the same strand as the original file
#flip strands 
cat ${Files}Hg19.bedfile | grep \- | cut -f4 > flipSNPs.txt
$plink  --noweb --ped ${Files}Red.ped --map hg19.map --flip flipSNPs.txt --recode --make-bed --out ${Files}Hg19
$plink  --noweb --bfile ${Files}Hg19 --recode --out ${Files}Hg19

run exampe

./liftover.sh myPlinkFile

angsd

[[angsd/][Download folder]]

plot admix results

Download

[[Rfun/plotAdmix_anders.R][Get the R script]]

or the more fancy one

[[Rfun/plotAdmix2.R][fancier plotter (ida)]]

to get the options type

Rscript plotAdmix_anders.R
Rscript plotAdmix2.R

plot LD region from plink file

plot the LD in a region. You need SNPmatrix working. If you cannot get SNPmatrix to work use Relate instead (ldsnp3 function) or if on pontus use

library(snpMatrix,lib="/home/albrecht/R/x86_64-pc-linux-gnu-library/2.15/")

R function: <include file="andersLink/ldplot.R" markup="example">

example

library(snpMatrix)
#snp info from plink files
pl<-read.plink("/space/anders/greenland/data/plink/datGRDKplus")
plB<-read.table("/space/anders/greenland/data/plink/datGRDKplus.bim")

#choose region
min.pos<-70e6
max.pos<-81e6
chr=1
minMaf<-0.05
minCallrate<-0.95
depth<-400 #depth of ld calculations (window size)

# calculatate maf and callrate
info<-col.summary(pl)

#chose SNPs
table(keep <- plB[,1]==chr&plB[,4]>min.pos&plB[,4]<max.pos & info$MAF>minMaf & !is.na(info$MAF) & info$Call.rate > minCallrate)

#estimate LD
ldinfo <- ld.snp(pl[,keep],depth=depth)

#plot LD (D')
ldplot(ldinfo$dprime,pos=plB[keep,4],main="LD (D')")

#plot R squared
ldplot(ldinfo$rsq2,pos=plB[keep,4],main="LD (r2)")

#change color palette
colR=colorRampPalette(c("white","grey","black"))(40)
ldplot(ldinfo$rsq2,pos=plB[keep,4],main="LD (r2)",colR=colR)

This example produces the plots: [[andersLink/ldpic1.pdf][pdf]]

API

#include "refFinder.h"
perFasta *pf = init("hg19.fa");
char refbase = getchar("chr20",130224101,pf)
//refbase now contains the reference base for chr20 at position 130,224,101
destroy(pf);

Remember to link with refFinder.o and -lz

g++ sampleProg.cpp refFinder.o -lz

bugs

1. do check if reference file doesn't exist.