ANGSD: Analysis of next generation Sequencing Data

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User:Thorfinn: Difference between revisions

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=ms and psmc stuff=
./msHOT-lite 2 20 -t 30000 -r 6000 30000000 -eN 0.01 0.1 -eN 0.06 1 -eN 0.2 0.5 -eN 1 1 -eN 2 2 -l
N0=theta/regionsize/4/u
30000.0/30000000.0/4.0/u=10000
from ms to realtime and realsize
tk are times from ms e.g. 0.01,0.06,0.2,1,2
alpha are sizes from ms e.g. 0.1,1,0.5,1,2
truetimes in years
4*g*N0*tk
from ms to real populationsize
alpha*N0
from psmc to realtime and realsize:
PA 4+25*2+4+6 0.026254885 0.003600825 21.895293401 5.782425186 2.106578468 0.452471596 0.479814497 0.401498376 0.367130222 0.302637758 0.336141050 0.585819704 1.405835053 2.909550136 4.044915857 4.137850214 3.776532973 3.123633638 2.466466904 2.013826351 1.792827385 1.762345431 1.839956170 1.916823191 1.921452795 1.902858530 2.051184729 2.597031978 3.508703366 4.125487051 6.375234256
Theta is third value.
N0=theta/2/u/s
from psmc time to realtime
2*g*N0*times
from psmc time to realtime
N0*lambda
=Wiki doc=
=Wiki doc=
#  check userdir is writable
# write how on website: Thetas,Tajima,Neutrality test needs documentation and examples files
# write how on website: Thetas,Tajima,Neutrality test needs documentation and examples files
#user custom class missing
#user custom class missing


=Code fix/cleanup=
=Code fix/cleanup=
#fix ./angsd0.591.smartcount/angsd -i angsd0.591.smartcount/DNK02.hg19.flt.sort.rmdup.realign.md.bam -out tmp -minQ 20 -minMapQ 30 -P 2 does nothing but crashes
#if(doMajorMinor==4&&refToInt[pars->anc[s]]==4) in analysisMajorMinor tsk 7april ?
# check that first line in .arg file is the command used
#check domajorminor for -domaf complains about it lagging
#fix -r problem if chromosome contains :
#if(doMajorMinor==4&&refToInt[pars->anc[s]]==4) in analysisMajorMinor
# don't quit program if a chr is not included in the -sites file
# don't quit program if a chr is not included in the -sites file
#check all getoptions stderr output.
#check all getoptions stderr output.
#-samglfclean not documented
#soap usage is not documented
# from morten shitoutput  
# from morten shitoutput  
<pre>
<pre>

Latest revision as of 23:13, 25 January 2018

ms and psmc stuff

./msHOT-lite 2 20 -t 30000 -r 6000 30000000 -eN 0.01 0.1 -eN 0.06 1 -eN 0.2 0.5 -eN 1 1 -eN 2 2 -l

N0=theta/regionsize/4/u

30000.0/30000000.0/4.0/u=10000

from ms to realtime and realsize

tk are times from ms e.g. 0.01,0.06,0.2,1,2

alpha are sizes from ms e.g. 0.1,1,0.5,1,2

truetimes in years

4*g*N0*tk

from ms to real populationsize

alpha*N0

from psmc to realtime and realsize:

PA 4+25*2+4+6 0.026254885 0.003600825 21.895293401 5.782425186 2.106578468 0.452471596 0.479814497 0.401498376 0.367130222 0.302637758 0.336141050 0.585819704 1.405835053 2.909550136 4.044915857 4.137850214 3.776532973 3.123633638 2.466466904 2.013826351 1.792827385 1.762345431 1.839956170 1.916823191 1.921452795 1.902858530 2.051184729 2.597031978 3.508703366 4.125487051 6.375234256

Theta is third value.

N0=theta/2/u/s

from psmc time to realtime

2*g*N0*times

from psmc time to realtime

N0*lambda

Wiki doc

  1. check userdir is writable
  2. write how on website: Thetas,Tajima,Neutrality test needs documentation and examples files
  3. user custom class missing

Code fix/cleanup

  1. if(doMajorMinor==4&&refToInt[pars->anc[s]]==4) in analysisMajorMinor tsk 7april ?
  2. don't quit program if a chr is not included in the -sites file
  3. check all getoptions stderr output.
  4. from morten shitoutput
h1 h2 h3 = 7 10 5  has less than 3 blocks. skipping
h1 h2 h3 = 8 10 5  has less than 3 blocks. skipping
h1 h2 h3 = 10 11 5  has less than 3 blocks. skipping
h1 h2 h3 = 7 12 5  has less than 3 blocks. skipping
h1 h2 h3 = 10 12 5  has less than 3 blocks. skipping
h1 h2 h3 = 10 13 5  has less than 3 blocks. skipping
h1 h2 h3 = 10 15 5  has less than 3 blocks. skipping
h1 h2 h3 = 7 10 6  has less than 3 blocks. skipping
h1 h2 h3 = 8 10 6  has less than 3 blocks. skipping
h1 h2 h3 = 9 10 6  has less than 3 blocks. skipping
h1…

Addtional methods and functionality

  1. make haploid 1dsfs
  2. simplefy filereading. from glf files
  3. fix -doFasta for single chromosomes.