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Preset reference populations can be altered. | Preset reference populations can be altered. To alter the reference populations one need to write comma separated populations to argument refpops. | ||
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Revision as of 21:15, 21 September 2014
This page contains information about the program called FastNGSadmix, which is a very fast tool for finding admixture proportions from NGS data of a single individual. It is based on genotype likelihoods. The program is written in R.
Installation
wget popgen.dk/?? OR Use shiny: popgen.dk/???
Run example
First download some example test files which has been generated on basis of data from the 1000 genomes project.
wget popgen.dk/??????/popfreq.RData wget popgen.dk/??????/beagle.gz
tralalala
YRI, CEU, Sardinian, WestGreenland, CHB, JPT, Sindhi, Papuan, Chukchi, Karitiana
Preset reference populations can be altered. To alter the reference populations one need to write comma separated populations to argument refpops.
HOME$ Rscript NGS_Admix_rscript.r beagle_GL='indi_genotypelikelihood.beagle' popfreq_RDATA='pop_freq_all.RData' outputfile='test'
Input Files
Input files are contains genotype likelihoods in genotype likelihood beagle input file format [1]. We recommend [ANGSD] for easy transformation of Next-generation sequencing data to beagle format.
Example of a beagle genotype likelihood input file for 3 individuals.
marker allele1 allele2 Ind0 Ind0 Ind0 1_14000023 1 0 0.941 0.058 0.000 1_14000072 2 3 0.709 0.177 0.112 1_14000113 0 2 0.855 0.106 0.037 1_14000202 2 0 0.835 0.104 0.060 ...